Structural and Biochemical Insights into the Function and Evolution of Sulfoquinovosidases

P Abayakoon; Y Jin; JP Lingford; M Petricevic; A John; E Ryan; J Wai-Ying Mui; DEV Pires; DB Ascher; GJ Davies; ED Goddard-Borger; SJ Williams

Journal article

Structural and Biochemical Insights into the Function and Evolution of Sulfoquinovosidases

P Abayakoon, Y Jin, JP Lingford, M Petricevic, A John, E Ryan, J Wai-Ying Mui, DEV Pires, DB Ascher, GJ Davies, ED Goddard-Borger, SJ Williams

ACS Central Science | AMER CHEMICAL SOC | Published : 2018

DOI: 10.1021/acscentsci.8b00453

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Abstract

An estimated 10 billion tonnes of sulfoquinovose (SQ) are produced and degraded each year. Prokaryotic sulfoglycolytic pathways catabolize sulfoquinovose (SQ) liberated from plant sulfolipid, or its delipidated form α-d-sulfoquinovosyl glycerol (SQGro), through the action of a sulfoquinovosidase (SQase), but little is known about the capacity of SQ glycosides to support growth. Structural studies of the first reported SQase (Escherichia coli YihQ) have identified three conserved residues that are essential for substrate recognition, but crossover mutations exploring active-site residues of predicted SQases from other organisms have yielded inactive mutants casting doubt on bioinformatic func..

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University of Melbourne Researchers

Douglas Valente Pires Author

David Ascher Author

Ethan Goddard-Borger Author

Spencer Williams Author

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Funding Acknowledgements

Australian Research Council (FT130100103, DP180101957), the European Research Council (ERC-2012-AdG-322942), the Ramaciotti Foundation and VESKI with additional support from the Australian Cancer Research Foundation and Victorian State Government Operational Infrastructure Support, NHMRC IRIISS Grant 9000220, are acknowledged. G.J.D. is supported by the Royal Society, and this work underpins recent Leverhulme Trust funding (RPG-2017-190). We acknowledge the staff of the Diamond Light Source (UK) for provision of I02 beamline facilities (proposal number mx-9948), and Amicus Therapeutics for a gift.